Studies Involving a Commercial Extract of Three Ballerina: ii- Evaluation of the in Vitro Effect on the Labeling of Blood Constituents of rats with Technetium-99m and on the Morphology of the Red Blood Cell.

The aim of this work is to characterize (electric conductivity and refractive index) a Three Ballerina (TB) and to evaluate its in vitro effect on the labeling of blood constituents with 99mTc and on the morphology of the red blood cells (RBC). Anticoagulated whole blood (Wistar rat) was incubated with a TB extract and the labeling of the blood constituents with technetium-99m (99mTc) was performed. Plasma (P) and blood cells (BC) were isolated and aliquots were also precipitated with trichloroacetic acid to separate soluble (SF) and insoluble fractions (IF). The %ATI in these samples was calculated. The morphology of the treated RBC showed no shape’s qualitative alterations. The TB extract was characterized with an electric conductivity of 1.35±0.04mSv/cm and refractive index of 2.21±0.15%BRIX. TB extract decreased significantly (p<0.05) the radioactivity distribution in the cellular compartment from 96.97±1.30% to 88.48±7.13%, and in IF-P from 74.29±4.12 to 14.26±5.73%. In conclusion, our data show some physical chemical parameters that could be suitable to characterize the preparation of an extract of TB. Moreover, substances present in the TB extract should probably have an effect on transport of the ions through the RBC membrane and/or should have redoxi properties and the stannous ion would decrease and could justify the effect on the fixation of the radioactivity on the plasma proteins. Moreover, although our experiments were carried out with animals, it is suggested precaution in the interpretation of the examinations that use labeled blood constituents in patients who are undergone TB extract.

Studies Involving a Commercial Extract of three Ballerina: I- Characterization and the Effect on the Binding of two 99mtechnetium-Radiopharmaceuticals on Blood Constituents.

We have determined some physicochemical parameters of an aqueous extract of Three ballerina (3TB) and its influence on the binding of the radiopharmaceuticals sodium pertechnetate (Na99mTcO4) and 99mTc-methylene diphosphonate (MDP-99mTc) on blood constituents. Whole blood of rats was incubated with a 3TB extract and with Na99mTcO4 or MDP-99mTc. Plasma (P) and blood cells (BC) from Wistar rats (control and treated) were separated. P and BC were precipitated with trichloroacetic acid (TCA) and soluble (SF) and insoluble fractions (IF) isolated. The percentage of incorporated radioactivity (%ATI) in each fraction was determined. The treatment not influenced statistically (p>0.05) the %ATI of both radiopharmaceuticals on BC and P compartments, as well as on IF-P and SF-P isolated by TCA precipitation. However, 3TB has altered the fixation of the 99mTc-MDP on the IF-BC and SF-BC. We are firstly reported physicochemical parameters (absorption spectrum, viscosimetry and phmetry) about an aqueous 3TB extract. Moreover, it is possible to speculate that an alteration would be not found on the biodistribution of the 99mTc-pertechnetate in the person that is undergoing 3TB extract. However, this would be found with the 99mTc-MDP.

Fibromyalgia and the relevance of the whole-body vibration exercises in vibratory platforms: a short review

Among nonpharmacological strategy to manage fibromyalgia, exercise (aerobic) has shown efficacy. Whole-body vibration (WBV) exercise has been proposed as a potential clinical intervention. WBV would induce increase in growth hormone (GH). An impairment of the hypothalamic-pituitary-GH-Insulin Growth Factor-1(IGF-1) axis has been implicated in the pathophysiology of fibromyalgia. This article aims to review the studies on exploring the relationship between WBV and fibromyalgia. Literature searches were performed in the PubMed database on 04/03/2010 using terms related to "pain", "whole body vibration" and "fibromyalgia". An important number of publications were identified with the term "pain" and in comparison, only a small number of articles were found related to "fibromyalgia". Three publications found with "whole body vibration" and fibromyalgia were analyzed.There are reports describing increase in serum IGF-1 following exposure to WBV in elderly patients. However, one randomized fibromyalgia trial revealed no changes in serum IGF-1 levels in women undergoing WBV. Due to the paucity of available, effective therapies for fibromyalgia, further studies that explore the relationship between the neuroendocrine system, fibromyalgia and WBV are merited.